This course covers theoretical and practical knowledge on plant, insect and animal cell and tissue culture technology and its application in bioprocess technology fields. The course provides detailed information such as research methodology, current findings as well as advantages and disadvantages of three different types of cell culture systems. The topics covered in this course include media preparation, sterile techniques, aseptic handling, initiation and routine maintenance of cells in culture, common contaminants of plant and animal cell culture, and understanding of some of the applications of cell culture technology. This course also covers the fundamental biology of stem cells and its applications including the integration of gene therapy, chemical approach, extracellular matrices and the latest reprogramming technology related to stem cells.